Insight on the bio-parameters affecting the formation of essential biomolecules and nutrient concentration, along with a monitoring and measurements system is of high demand. It becomes crucial to utilize a real-time process analysis system to result in a high-quality yield for any bioprocess. Control schemes are being implemented to enhance productivity and product outcome without compromising process efficiency. Bioprocesses play a vital role in the field of biotechnology and are widely preferred in many industrial sectors. Monitoring bioprocesses is highly essential because low time intervals do not cause many variations in the values of the bioprocess. This work presents the development of a Graphical User Interface for Bioprocess parameters monitoring such as temperature, pH, and dissolved oxygen for a Fermentation process using LabVIEW.